FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2788237
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11350.052.233721
TCTGTCG11800.043.3099678
TCGCTCT14100.039.817984
CTGTCGC15800.035.5344059
TCTCGCT15050.034.9126052
GTCTTGC24600.034.810681
CTCGCTC14750.034.647353
CGCTCTG16050.031.8410265
GTGTTAG15000.030.740511
GTCTCAC31800.029.000481
GTGTTAC10800.027.7857341
GGCGTAC1357.649997E-427.1080341
GTCTTAC10600.026.9290181
GTTTCAC36200.025.6498533
GTCTAAC5900.024.8107411
GGGTTTC38900.024.6481021
TTGCTCT33650.024.5989484
TCACTCT37000.023.928044
TCTGTCA34250.023.5379688
GTTAGCC14300.023.1539733
Produced by FastQC (version 0.11.5)