FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3236784
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12150.037.928741
TCTGTCG14700.036.2329678
TCGCTCT13000.033.219834
CTGTCGC18800.031.776779
GTCTTAC12800.031.4309041
CTCGCTC14600.029.5793023
GTCTTGC29500.029.5072751
TCTCGCT14950.028.8863622
GTATTAC6450.027.2179661
GTATTAA9500.025.4094051
GTGTTAT11050.025.1550671
GTATAAC5000.024.8704191
GTCTCAC37100.024.8428151
GTATTAG8450.024.2384871
GTATAGT8300.023.7952271
CGCTCTG17300.023.2986875
GTCCTAC9700.022.6231821
GTGTTAG20050.022.6194211
GTGTTAC13500.022.2154061
CTACGCT3254.3157343E-822.1465534
Produced by FastQC (version 0.11.5)