FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227511
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11900.043.562488
CTGTCGC15150.035.167889
GTCTTGC27900.032.9386141
GTATAAC6400.029.3967421
GTGTAAA17900.024.6594071
GTATTAA9450.024.5032651
GTGTAAC10050.024.4804081
GTGTTAC12850.023.6511341
GTATAAT10100.023.642771
GTATTAC8600.023.5594641
GTCTCGC11100.023.46851
GTGTTAT12650.023.4530411
TGTTAGC19050.023.4429472
GTCTCAC35650.023.3423251
GTGTTAG21550.023.1690181
TCGCTCT13200.022.9162624
TATACTG10750.022.77045
TATACTT9550.022.616125
GTATATA12750.022.1340181
GTTAGCC17700.021.975363
Produced by FastQC (version 0.11.5)