FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227511
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA1002.570363E-643.8967171
TCTGTCG12500.040.8871578
GTCTCGC13950.038.8095251
CTGTCGC16650.034.5871169
GTCTTGC29600.031.8844431
TCGCTCT15750.031.0789954
CGCTCTG16250.027.4644075
TCTCGCT17600.026.9929082
CTCGCTC18000.025.9943773
GGTATAC6650.024.2037051
GTATTAA9400.024.127631
GTCTCAC34750.024.0010831
GTATTAG10400.023.2146111
GGAGTGC70250.023.0551575
GTTTCAC48500.022.5600723
CTTGTCG3203.6579877E-822.4951349
GAGTGCA77800.022.2059946
GTGTTAC12550.022.1523951
GTATTAT12150.021.6773911
CTTGCTC39550.021.6590353
Produced by FastQC (version 0.11.5)