FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983246
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.041.2318238
GTCTTGC25100.034.3019681
TCGCTCT13800.033.402284
CTGTCGC15150.033.265999
GTCTCGC13350.033.0593531
GTATTAT8750.028.1136151
CTCGCTC15400.027.128983
TCTCGCT15750.026.983462
GTATAGA7800.025.9722291
CGCTCTG15600.025.8446835
GTATTAA7900.025.6434631
ATACCCG2601.2218152E-724.931274
GTGTTAA11900.024.9277271
GTATAGT9050.024.7832871
GTGTAAA16450.023.3107221
GTGTTAT10900.023.2320381
TTGCTCT34450.023.2064634
GTGTTAC11850.023.201231
GTCTCGA24950.022.2191756
GGGTTTC46850.021.9292871
Produced by FastQC (version 0.11.5)