FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983246
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10400.040.0797271
TCTGTCG12600.034.276458
TCTCGCT15000.029.2701262
TCGCTCT13550.029.2167074
GTCTTGC25650.027.939711
CTCGCTC14700.027.9103553
GTATTAC6900.027.555451
CTGTCGC16200.027.1037859
GTCTCAC34450.026.3218041
CGCTCTG14550.024.2404635
CGTTTCG1801.597308E-423.9935159
GTATTAG8300.023.78861
GTATTAA8600.022.9587671
TATACTG9450.022.8505845
GGCTAGA17550.022.50091
GTCTTAC10750.022.448571
GTATAGG7250.022.190541
TCACTCT39350.022.1334194
GGGTTTC45300.021.954521
GTGTTAC11100.021.7407341
Produced by FastQC (version 0.11.5)