FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980395
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10150.037.5945668
GTCTTGC26250.031.9622691
GTCTCGC12500.030.6672441
CTGTCGC13700.029.9550159
GTATTAA8600.027.7539231
GTCTTAC11350.026.7646981
TCGCTCT14450.025.9137884
GTGTTAG18300.025.690421
GTACTAT6000.025.3149431
GTGTTAC10900.024.5518441
TAATACT8300.024.2926754
TCTCGCT15150.023.2928472
GTCTATG10100.022.9159321
CGCTCTG14250.022.7355585
GTATAAC6050.022.7147061
GTGTAAG14700.022.6333791
TTACACT13100.022.5376154
CTCGCTC15100.022.41613
GTCTAGA11950.022.394571
TTACTCT16900.022.1570554
Produced by FastQC (version 0.11.5)