FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980395
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12550.041.870498
GTCTCGC11550.038.6287841
GTCTTGC24150.033.9206431
TCGCTCT13050.033.6460154
CTGTCGC16200.032.4367039
CTCGCTC12950.031.6824993
CGCTCTG14600.029.5809925
TCTCGCT14450.028.8913042
GTACGGA2104.6220703E-727.8633841
GTCTCAC32850.025.38241
GTGTTAC11950.025.0945341
GGTATAC5050.024.6218531
GTGTTAG19800.024.3804631
GTAGCGT2401.7031889E-623.993476
TTGCTCT37100.023.6701094
GCGTAAT1952.4650356E-422.5050411
GTCTTAC10650.021.9767531
GTATATA12100.021.7610741
GTTACCC9450.021.327533
GTGTAGC14750.021.3225751
Produced by FastQC (version 0.11.5)