FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417305
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10350.036.8599178
TACGACT1551.550985E-632.5069474
CTGTCGC13550.030.8123979
GTCTCGC10600.030.3639581
TCGCTCT9450.029.7058934
GTCTTGC23650.028.1463321
GTGTAAC7700.026.599831
GTATTAA8050.026.3520071
GTGTTAG14750.026.2842391
GTATTGG9000.026.0087221
GTCTTAC9650.024.256841
GTGTAAA16700.024.0911641
GGGTTAT6750.023.841331
GTATTAT8150.023.3360481
AAGTCCG2202.3582144E-522.902625
GTCTCAC29450.022.6030831
TCTCGCT12450.022.5464482
CTCGCTC12700.022.103993
GTATTAG8350.021.9010581
GTATTGT8750.021.735861
Produced by FastQC (version 0.11.5)