FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668375
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12200.034.811128
CTGTCGC14500.030.7792229
GTCTCGC11950.030.0032671
GTACGCC1504.6224304E-528.7923873
GTCTTGC25200.028.165121
TCGCTCT12650.027.8819584
GTACTAC4550.027.3387051
GTCGTAC1357.662411E-427.1004581
GTATTAG10150.026.673261
GTCTTAC12000.026.2196921
GTGTTAG16600.026.0066451
TCGATCT9300.023.9945559
GTACTAG6150.023.7955231
GTACTAA6550.023.459481
CTCGATC9350.023.0959328
GTTAGCC13750.023.0339093
GTACCGC1600.002062586122.8660131
GGGTACG2252.4712805E-522.7643831
TGTACGC1902.3041884E-422.7304062
GTCTCAC31000.022.659481
Produced by FastQC (version 0.11.5)