FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375907
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10550.040.938378
GGTATAC4300.037.4138221
GTCTCGC10600.035.873631
GTCTTGC22050.035.8173141
TCGCTCT11550.034.9002574
GTGTTAG16900.033.7509271
CTGTCGC13650.032.695719
GTATTAG7600.031.7525221
CTCGCTC13350.029.6554183
GTTAGCC13350.027.498663
CGCTCTG13350.027.498665
GGGTACG2404.8550646E-827.422631
TCTCGCT14300.026.6780012
GTCTTAC8850.026.4414061
GTGTAAT9750.026.2507231
GTGTAAA14700.025.8680571
TTGCTCT31500.025.8220374
CTACGTA1701.0832653E-425.405879
GTACTAT6800.024.7341371
GTATAGA7200.024.3756711
Produced by FastQC (version 0.11.5)