FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3663262
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14100.039.8294878
GTCTTGC35450.036.1530381
CTGTCGC18300.034.229259
TCGCTCT16550.031.323884
GTCTCGC14800.030.6492521
CGAATAC1753.9867627E-628.8005683
TCTCGCT16900.026.8407672
TTGCTCT44950.026.4298884
GTATAAT11550.026.1823351
GCGTAAC1959.244897E-625.8466641
GTTTCAC53150.025.6035163
CTTGCTC43650.025.5675143
CTCGCTC18600.025.5488913
GGGTTTC57150.024.69341
GTGTTAG26500.024.1816081
TCTGTCA46250.024.1295478
GGAGTGC87800.023.8637965
GAGTGCA95750.023.8368956
GTATTAA9500.023.4951971
CGCTCTG19750.023.3321065
Produced by FastQC (version 0.11.5)