FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604443
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC855.5660632E-542.353571
TCTGTCG9850.040.2028668
GTCTCGC10150.036.8872451
TCGCTCT11550.033.6628384
CTGTCGC12550.032.1273469
GTCTTGC22950.030.7455541
TCTCGCT12650.029.0280972
GTATTAA7650.028.2357121
GTACGAC1306.7529676E-427.6927173
CGCTCTG12800.027.5629085
CTCGCTC12800.026.4378913
GTACTAA5500.026.1822051
GTACGGA2001.1249851E-525.2003731
GGGTAAC7200.025.0003721
GTATTAT9400.023.7450311
GTGTTAG17350.023.654531
GTCTTAC10200.022.588571
CTCGATC10250.022.477958
TCTCGAT9950.022.432067
GTCTCAC27950.022.411781
Produced by FastQC (version 0.11.5)