FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604443
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11250.036.510711
TCTGTCG11550.036.1543248
TCGCTCT12300.032.193774
GTCTTGC21800.031.7331141
TAGTCCG950.004892467530.3145165
CTGTCGC16850.026.4914689
GTCTCAC27450.025.7265571
TCTCGCT15900.025.3637542
CTCGCTC14500.024.3346823
CGCTCTG13350.023.729345
GTATTAA8300.023.4414041
TAATACT9050.023.0708544
GTGTTAG17000.022.8898411
GTCTAGC11550.021.8365481
GTATTAC6700.021.5106321
GTACTAA6750.021.3512941
ATACCGA1700.003221597821.1755816
TCACTCT34850.021.0722874
ACACCGT4151.193257E-920.8184036
GTCTAAT6600.020.7447221
Produced by FastQC (version 0.11.5)