FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840627
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11250.037.7597778
GTCTCGC10700.032.9717671
TCGCTCT12300.032.7802934
CTGTCGC14250.027.7893079
TCTCGCT13800.027.6520082
CGCTCTG14100.025.5317615
CTCGCTC13550.025.5053843
GTCTTGC22100.025.0858231
GTCTCAC26350.024.0453971
GTATAAA9650.023.1293931
GTCTTAC8900.021.8425671
GTACTAG4751.2732926E-1121.2209261
GGCTGGA88000.021.1089631
GGAGTGC62700.021.0142295
GTATAAC4851.8189894E-1120.783381
GAGTGCA68250.020.2548226
GAACCGT2857.544064E-620.2104056
GTATTAG8200.020.1951
TTCGCTC4301.8571882E-920.0929013
GTGTTAC9400.019.9147761
Produced by FastQC (version 0.11.5)