FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840627
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12750.037.2689258
GTCTCGC12000.035.4252321
GTCTTGC22950.032.9646571
TCGCTCT14150.029.5110514
CTGTCGC16300.029.1520739
TCTCGCT15600.025.3933162
CTCGCTC14500.025.3292483
GGTATAC3151.1132215E-925.1607781
CGTTTCG1801.5948394E-423.998939
CGCTCTG14850.023.7565165
GGAGTGC66000.023.45355
TTGCTCT32750.023.3027764
GAGTGCA71100.023.0875786
GTTCCGA2552.8600934E-622.6043381
CTGGAGT75450.022.3345283
GGCTGGA88300.021.949951
GTGTTAA11250.021.775511
CTTGCTC31000.020.675143
GTCTCAC30150.020.5519521
TGGAGTG82850.020.1608374
Produced by FastQC (version 0.11.5)