FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2539170
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10850.047.816841
TCTGTCG10350.040.3457578
TCGCTCT11950.037.956214
TCTCGCT13400.037.08662
GTCTTGC20750.034.726421
CTCGCTC13900.033.1572153
CGCTCTG13900.031.0775435
CTGTCGC14800.030.6470789
GTGTTAC9500.030.3399261
GTATTAA7200.029.0230871
GTTTCAC34350.025.3671383
GTCTTAC9800.024.9994771
TTGCTCT31200.024.6910444
GGGTACG2401.6872709E-624.0191081
TAATCCG1801.5948465E-423.998775
GTGTAAA16700.023.7314531
GTGTTAG17650.023.6788941
GGGTTTC40050.023.5693111
TTCACCG7450.023.1934415
CTATTAC6550.023.1023461
Produced by FastQC (version 0.11.5)