FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262365
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9000.048.799958
GTCTCGC10150.042.561531
GTCTTGC22550.040.8691371
CTGTCGC11850.037.6708459
TCGCTCT11950.034.9455684
TCTCGCT12700.031.181072
CTCGCTC12500.031.1039663
TTGCTCT31950.030.6478544
CGCTCTG13050.029.793075
CTTGCTC28700.028.0975323
GTCTCAC25150.027.7693541
GTGTTAG16600.027.7590071
TCTTGCT31200.027.692282
GTCCTAC6150.026.92681
ACCGTGT7800.026.7692038
GTATTAA8200.026.3414341
GTATAAC5100.025.4117371
TTCACCG6050.024.991715
GGTATAC4101.8189894E-1224.5853391
GTATTAG7700.024.3116631
Produced by FastQC (version 0.11.5)