FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262365
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10600.047.5774731
TCTGTCG10250.036.525078
GTATCGC1251.321454E-534.5820241
TCGCTCT11700.034.4598774
TCGCACT2800.033.4269644
CTCGCTC13050.032.558793
TCTCGCT13300.031.94892
CGCTCTG12050.030.471565
CTGTCGC13600.030.1750189
GTGTTAC10950.029.6078991
GTCTTGC21200.029.5660021
TACTGCG2802.8558134E-1028.2843537
GTACTAC4600.028.1918681
GGGTTTC39550.026.2316271
GTCTCAC25700.025.7907471
GGCTAGA13150.025.2023661
GTACTAG4900.024.9955121
ACCATGT29150.024.9456258
GTATAGT6150.024.6010361
GTATTAT7100.024.353541
Produced by FastQC (version 0.11.5)