FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868160
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10550.047.0899288
GTCTCGC12350.041.97561
TCGCTCT13550.034.538664
CTGTCGC14300.034.2376759
GTCTTGC25550.030.7161641
TCTCGCT15500.028.7999252
CTCGCTC15100.028.1323783
CGCTCTG16150.027.1949775
GTCTCAC30900.025.1649861
GTGTAAC7450.025.1274531
TTGCTCT33800.024.0709444
TTATACT8100.023.9999394
GTTTCAC38850.023.1659623
CTCGATC10700.022.8784458
GTGTAAA15750.022.8570821
TCTCGAT10400.022.153797
TCGATCT10800.021.9999459
GTTACCC9500.021.978893
GTCTTAC11600.021.7240831
GTATAAG8650.021.6415631
Produced by FastQC (version 0.11.5)