FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868160
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11850.039.5318571
TCGCTCT13700.035.7356154
TCTGTCG12150.033.183698
GTCTTGC24400.032.4904021
CTCGCTC14450.029.9021423
TCTCGCT14900.029.4870012
CGCTCTG15200.028.4197675
GTATTAG10850.025.9052091
GGGTTTC42250.025.075111
GTATTAT7600.023.707111
CTGTCGC16850.023.5004229
GTTTCAC39900.023.2828253
GTCTCGA27250.022.9861166
GGTATAC4403.6379788E-1222.9312421
GTATAAC5050.022.8339391
CTCGATC10450.022.7358158
GTATACG1600.002252743822.5217551
GTGTAAG12950.022.2608871
TTGCTCT36450.022.122464
GCGTAGT4359.094947E-1121.5380481
Produced by FastQC (version 0.11.5)