FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512549
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11400.039.1577031
TCTGTCG8550.036.210358
GTACCGA800.002107633735.9998251
TCGCTCT10950.032.8765534
TCTCGCT12350.030.8986382
CTGTCGC12150.030.8146679
CTCGCTC12150.029.0368983
GTCTTGC18550.026.0052641
GTCTCAC24150.024.7452221
CAATCCG1801.5943375E-423.9998865
GTACTAA4154.9112714E-1122.554111
GAGTGCA59950.022.2184076
ACCGTGT7200.021.9998958
CGCTCTG13100.021.9846275
GGAGTGC55850.021.6579075
GTATAAG7850.021.095441
TCTCGAT9800.020.571337
GTCTTAG9800.020.571331
GTCTCGA23650.020.3973666
GTATAGG7100.020.2815911
Produced by FastQC (version 0.11.5)