FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512549
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10400.033.257251
TCTGTCG12100.032.725988
GTCTTGC21600.031.6919041
TCGCTCT10800.031.3320964
GTACCGG2603.97813E-927.7143781
CTGTCGC15900.024.9046769
TTGCTCT28400.024.5905784
CTCGCTC12800.024.1908783
GTCTCAC25500.023.1713941
TCTCGCT14150.022.9034582
GTGTTAG15900.022.6595551
CGCTCTG13800.022.4338955
GAGTGCA57050.021.9588176
GGAGTGC54550.021.5133555
CTTGCTC29050.021.0700683
GGCTAGA12750.020.910771
GTCCTAC6550.020.9021421
GTGTAAG10700.020.8764381
GTACTGA7100.020.2978551
GTCATAC5353.6379788E-1220.2030051
Produced by FastQC (version 0.11.5)