FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809444
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12750.042.3872871
TCTGTCG11450.036.470018
TCGCTCT14250.036.3773464
ACGTACG1153.2954165E-431.3029734
GGTATAC6250.029.9762921
CTCGCTC15800.029.6258053
GTCTTGC24600.029.2920281
TCTCGCT17350.028.6424332
CGCTCTG15950.028.4376185
CTGTCGC16400.025.4622949
GTGTAAC10400.024.9432891
GTCTTAC11100.024.0194631
GTATTAT9100.023.7555141
GTATAAC6500.023.2804031
GGAGTGC63300.022.7478145
TACACTG17150.022.6695585
TCGAGCG1600.002265997522.499019
GTACTAA6100.022.4444161
GTGTAAT11900.022.4047091
GTTTCAC40600.022.1716423
Produced by FastQC (version 0.11.5)