FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297179
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10150.039.0147178
GTCTCGC9500.038.6550981
GTATACG1501.1972134E-633.6021421
GTCTTGC19050.033.2619631
GTATAAG5150.030.7592451
CTGTCGC12450.030.0722459
TCGCTCT11750.028.7999554
GTATTAG6700.027.942081
GTTCGTA1050.00797282427.430321
GCGTAAT1050.00797282427.430321
TCTCGCT12000.026.9999582
GGAGTGC56500.026.7610235
GAGTGCA59150.026.657616
CGCTCTG12600.026.2856735
GGTATAC4450.025.8892921
GTGTAAT8650.025.8051171
GTACCGC1400.001041062125.7142476
GTCTTAC7900.025.5206151
CTGGAGT66200.024.7975463
GTATAGC4400.024.547021
Produced by FastQC (version 0.11.5)