FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556879
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10700.037.6803478
GTCTCGC11300.035.1317981
CTGTCGC14150.029.510889
TCGCTCT12150.027.8509944
GTCGTAA1306.6557224E-427.7614861
GTATTAG8850.026.9145261
GTCTTGC24100.026.6556361
TAATACT8450.026.4133874
CGTTTCG1658.832564E-526.1804989
GTATTAA7750.026.0778871
TCTCGCT13300.023.8725012
CGCTCTG13000.023.814655
GTGTATA12150.023.7629181
GTCTCGA24450.023.2626326
TCGATCT10000.023.0388379
GTTTCAC39000.022.94323
TTACACT13250.022.821944
TTGCTCT31600.022.7841074
CTCGATC9950.022.431038
CCGTGTT8750.022.2160249
Produced by FastQC (version 0.11.5)