FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556879
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11850.038.8860638
TCGCTCT13150.037.7794574
GTCTCGC12800.036.0013961
GTCTTGC23250.031.5883241
CGCTCTG14050.030.7473225
CTGTCGC15250.030.2163879
CTCGCTC15950.028.8902743
TCTCGCT16050.028.7102742
CGTTTCG1400.001041086725.7142779
GGGTTTC43700.024.5501541
TTGCTCT31700.024.3028324
GTTTCAC40100.024.2393953
GTGTTAC8750.023.8637851
GTATAGG6050.023.8025781
GTATATA11200.023.7866381
GGAGTGC58800.022.8979535
GAGTGCA65850.022.742596
GTATTAG7700.022.4424321
GTCTTAC9450.022.0960981
GCGTTAA1650.00270800521.8190291
Produced by FastQC (version 0.11.5)