FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883319
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8300.046.8445431
TCTGTCG8700.038.8964928
TCGCTCT10150.036.8866424
CGCTCTG11650.034.609395
GGCGTAC1501.1974225E-633.6008451
TCTCGCT10300.033.553352
GTATTAA4600.031.305131
CTCGCTC11250.030.7199543
GTCTTGC16450.030.2013661
CTGTCGC11400.029.6841689
TCTATCG1555.768693E-527.8709268
GTGTTAG12400.026.1296861
GTACTAT4150.026.0247481
GTTTCAC25500.025.694083
GTATAGC3951.8189894E-1225.5196291
GTGTTAT6950.024.8639341
GTATAGG4650.024.7748151
GGTATAC3201.3460522E-924.750621
GTATAAT4400.024.546071
TATACTG5000.024.4799655
Produced by FastQC (version 0.11.5)