FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316304
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9350.047.7411848
GTCTCGC10050.035.1818921
CTGTCGC13400.033.3119439
GTCTTGC22050.031.0888161
TCGCTCT10950.030.2452244
GGGTTTC34400.028.3181251
TCTCGCT12200.027.7904552
TTCACCG5800.026.06785
GTATTGC5900.025.6836341
GTTTCAC32400.025.6041563
GTCCTAT7050.025.5882191
CTCGCTC13950.024.8213083
TTGCTCT27550.024.0424844
GTGTTAC8750.023.9154821
GTGCTAC8800.023.7795981
GGTTTCA35000.023.0838172
GGTATAC4154.7293724E-1122.6039541
GTATTAA6100.022.4756851
GTATAAT6100.022.4756851
GTGTTAG16850.022.2685831
Produced by FastQC (version 0.11.5)