FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316304
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11300.045.8759161
TCTGTCG10200.043.7645238
TCTCGCT13350.038.8312952
TCGCTCT14300.035.74814
CTCGCTC15650.034.0445863
GTCTTAC8100.033.7776341
CTGTCGC14200.033.464659
GTCTTGC22000.033.0544051
CGCTCTG15900.032.6036385
GTATTAT6850.030.4816231
GTGTTAG16750.026.6506351
GTGTTAC8400.026.5713161
GCGTTAC1400.001041080625.7141781
GTCTCAC27500.025.6580751
GTTTCAC33850.025.0988623
GGTATAC4600.025.0433731
TTACTCT12650.025.0433714
ATACTGA10950.024.3286656
GTATAGC5400.023.9998991
TCTGTCA27600.023.7390338
Produced by FastQC (version 0.11.5)