FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092701
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8500.048.2802478
CTGTCGC11150.038.7427069
GTCTCGC10600.036.7478871
GTCTTGC18650.035.9706731
TCGCTCT10550.035.4866034
TCTCGCT12350.029.7855822
CTCGCTC12500.029.4281563
GTGTTAG15100.028.662811
GGTATAC4100.028.1501431
GTACCGA1306.679697E-427.744131
CGCTATC1306.683437E-427.7414722
GTATAGG5100.026.8737261
GTCTCAC25400.026.6955321
TTGCTCT26850.026.5463284
GGGTTTC34750.026.1553271
GTTTCAC31100.025.9752943
GTTAGCC11500.025.715143
CTGTTGC23000.025.0423859
CGCTCTG13400.024.715345
GTATAGA5950.024.2469711
Produced by FastQC (version 0.11.5)