FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042241
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8400.042.8551528
GTCTCGC9600.042.836111
TCGCTCT10350.036.867854
CTGTCGC10950.036.820219
GTCTTGC20050.035.982561
TCTCGCT11150.032.989362
CTCGCTC11400.031.0005823
GTCTTAC8000.030.661641
CGCTCTG11600.029.791725
GTATTAG5600.028.3426911
TATACTG7000.025.7130935
TACTGCG2001.1253638E-525.1988327
GTCTCAC23600.024.7616421
GTACTAA5050.024.2864461
GTGTTAC8450.023.9060441
GTATAGG6400.023.6725881
TTGCTCT28500.023.4936474
ACCGTGT8300.023.4205998
GTGTAGT13050.023.2190911
GTATAAG7150.023.2074931
Produced by FastQC (version 0.11.5)