FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042241
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8550.045.47781
TCGCTCT10250.035.121694
TCTGTCG7400.035.0267688
TCTCGCT10400.033.2305222
TACGGTA1453.6612437E-529.792882
TAATACT5200.029.0767064
CGCTCTG12100.028.561775
CTGTCGC10400.028.3844039
CTCGCTC12200.028.3276563
GTCTTGC20400.028.237851
GTACGGT1856.137363E-627.2457081
GTCTCAC25550.027.0552851
GTATAGG5350.026.9183221
GTATAGA6050.026.1841871
GGTATAC3850.026.1841851
TTATACT7450.026.0937654
TCTGTCA28250.024.721948
GTCTTAC8550.024.4232621
GTATTAA5650.024.214581
GGTCTAG6700.023.6439291
Produced by FastQC (version 0.11.5)