FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2905240
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13050.043.032528
GTCTCGC13950.035.6935921
GTCTTGC25900.033.1560331
TCGCTCT14400.032.9984934
CTGTCGC16850.032.9005749
TCTCGCT16900.029.0310462
GTGTAAT12900.027.4108051
GGTATAC6100.027.209051
GTGTTAG19750.027.0383421
CTCGCTC16800.026.627043
GTGTTAC13700.025.8101731
GTATTAG8700.025.7133031
GTATAGC6500.025.5346471
GTCTTAC11000.024.9290851
CGCTCTG18450.024.5842425
GTCTCAC38050.024.4652961
TTGCTCT37700.023.871594
GTGTAGA13850.023.4465051
CTTGCTC35700.023.4439223
TAATACT9350.023.100554
Produced by FastQC (version 0.11.5)