FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949603
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13550.039.855081
TCTGTCG14800.037.9459278
TCGCTCT14950.033.712364
CTGTCGC18700.031.1871519
GTCTTGC26750.030.6861781
GTATTAC6400.029.2519681
TCTCGCT17350.029.0489792
GTCTTAC12100.027.9688261
GTGTTAG20950.027.1521851
GTATTAG8550.026.9491841
CTCGCTC17600.026.1818073
TAATACT9400.026.0425424
CGCTCTG17300.025.3872725
GTGTAAC8900.024.2712971
CTATGCG2401.699158E-623.9999899
GTCTCAC35150.023.7626251
TATACTG6800.023.2941075
GGGTTTC48700.022.4737931
CTGTTGC35000.022.4228469
GGTATAC6150.022.24541
Produced by FastQC (version 0.11.5)