FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110583
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12600.042.8570948
GTCTCGC12700.041.9547351
CTGTCGC17000.033.8823139
GTCTTGC25950.031.631551
TCGCTCT14150.031.0388374
TCTCGCT16750.030.5193652
CTCGCTC16450.027.5744383
GTATTAC7900.027.3430611
GTATTAA7900.025.5201911
CGCTCTG16450.025.385995
GTACTAG6550.025.2836341
GTGTAAC10450.024.8049961
GTATTAT9750.024.3703781
GTGTAGT19900.023.8805221
GTATCGC1851.9192215E-423.3524511
GTACTAC5250.023.3153821
TCTGTCA37900.023.1767568
TTGCTCT38500.023.0025734
GTCTCAC37100.022.9013481
GTGTTAC11950.022.8964771
Produced by FastQC (version 0.11.5)