FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2746042
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11400.032.208968
GTCTCGC12200.029.5713791
GTATTAC6150.028.1577221
GTACCGA1306.669725E-427.75161
CTGTCGC15800.027.3404459
GTCTTGC25500.027.163921
GTATTAG9250.026.5215281
GTATAAT7650.026.4093671
GTATAGG7000.025.7693441
GTACTAT5650.025.5412961
TCGCTCT12950.025.0180914
GGTATAC4700.024.5631181
GTGTTAG18800.023.795521
GTATTCG2151.9376452E-523.4920541
GTATAGC5950.023.0408251
GGCGTAA3153.0189767E-822.9060821
TCTCGCT14050.022.5934352
GTATTAA6500.022.201281
GTCTACG2603.3434299E-622.201281
GTCTCAC32550.022.1671771
Produced by FastQC (version 0.11.5)