FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896323
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13900.039.8987121
TCGCTCT14000.038.0565264
TCTGTCG13000.037.6609278
GTCTTGC27750.035.298711
GTCTTAC11250.032.011091
TCTCGCT16750.031.387272
CTCGCTC17200.030.5576463
CGCTCTG17450.030.5324575
CTGTCGC17700.027.2542679
GTGTTAG20000.027.0093561
GTATTAC5800.024.8361871
TTGCTCT37700.024.8271834
GTCGTAC1500.001556573423.9996133
GTCTCAC34650.023.9043831
GAGTGCA79650.023.1408676
GTATATA11600.022.9734731
GTCTAAC6000.022.80791
GTATAAG8250.022.6987721
GGAGTGC72950.022.502725
TCTGTCA37950.022.3869888
Produced by FastQC (version 0.11.5)