FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3045989
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12850.038.6601458
GTCTCGC12600.035.4360771
GTATTAC6900.029.2235811
TCGCTCT14400.028.9994
CTGTCGC18350.028.6420659
GTCTTGC27950.026.02341
TCTCGCT16200.025.7823932
GTCTCAC36900.024.98091
GTGTTAG20650.024.4119511
CGCTCTG18300.023.9991725
GTCTTAC10850.023.894461
CTCGCTC17050.023.647283
GTGTAAT11350.023.4763371
GTATAGA8900.023.4656451
CCTATCG1851.9202699E-423.3505469
TAATACT9200.022.694874
GAGTGCA75650.022.1750476
GTGTAAA17550.022.1585391
TCCGGTA1952.7385307E-422.1578122
GGAGTGC69500.022.0654265
Produced by FastQC (version 0.11.5)