FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3168280
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13100.046.1667448
GTACTAG6500.035.4586681
CTGTCGC17600.033.9536679
GTCTTGC29100.032.9188351
GTCTCGC13850.030.6823081
TTCACCG10000.026.639315
GTGTTAC13100.026.3909931
GTATAAG7750.026.0220871
GTCTTAC11450.025.7907621
TCGCTCT16600.025.5896994
GGTATAC6250.025.3529471
TTGCTCT38400.025.124354
GTACTAC6100.024.7956351
GTCTCAC36750.024.4984441
GTGTAGT16900.024.2925971
GTGTTAG19800.023.2809431
TCTCGCT18800.022.9842952
GTACCGG3153.0770025E-822.8652131
CTTGCTC39100.022.833173
GTATAAT7000.022.6365591
Produced by FastQC (version 0.11.5)