FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3198352
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13400.046.7434238
GTCTCGC14800.043.463851
TCGCTCT16250.039.4314424
CTGTCGC18800.036.3807649
CTCGCTC17600.034.4342163
CGCTCTG17750.033.2599455
TCTCGCT18300.031.9343072
GTACTAC5400.029.446171
GTGTTAG21650.028.3766351
GTCTTGC31450.028.2673021
GTCTTAC13500.026.7692451
GGTATAC6000.026.5015531
GTATAGT8100.024.9846291
TTACACT14300.024.1663594
ACCGTGT9650.023.8741958
GTGTAAA17600.023.8185441
GTGTTAC12250.023.600641
GTATTAG11950.022.983471
GTCTCAC38100.022.7643971
GTATAAG8600.022.6916051
Produced by FastQC (version 0.11.5)