FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3198352
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14950.040.9354328
TCGCTCT15050.035.401114
GTCTCGC14600.035.0243231
GTCTTGC28050.034.919681
CTGTCGC19000.032.5886579
CTCGCTC17350.028.6332913
TCTCGCT17800.028.3205412
TACACCG2105.304628E-727.4278855
GTATTAC6200.025.5561391
CGCTCTG18600.025.5477495
GTATTAT9350.025.4194761
TAATACT9700.023.7519864
TTGCTCT40500.023.6438524
GTCTCAC35950.023.6399921
GTATAGA8100.023.1181241
GTCTTAC12550.022.955171
GGGTTTC52750.022.801231
TTACACT15500.022.7607214
GTATTAA8900.022.6585561
GTTTCAC48450.022.5876733
Produced by FastQC (version 0.11.5)