FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147366
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14900.042.1979831
TCTGTCG13250.036.9487468
TCGCTCT15450.036.8133434
GTCTTGC27800.032.4955561
TCTCGCT18550.031.8980642
GTCGTAA1402.808813E-530.9729081
CTGTCGC16650.030.700889
CTCGCTC20000.029.2246573
GTCTTAC12100.028.669141
GTATAAC4800.028.606921
CGCTCTG19150.027.8207995
GTATAAT7900.026.5295351
GTCTCAC36850.025.4955621
GTATTGA8750.024.7783261
GTATTAT8400.024.090041
TCTGTCA37350.023.5166748
TTGCTCT39950.023.4278934
GTTACAC8850.022.8301723
TATACTG9250.022.571635
CTGTTGC33050.022.0017179
Produced by FastQC (version 0.11.5)