FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2633189
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10900.036.329838
GTCTCGC11800.036.013921
GTCTTGC25300.035.0174871
TCGCTCT12200.030.097994
GTATTAG9500.027.2947621
GTCTTAC10350.027.1409261
GTATACG2405.6237695E-827.010441
CTGTCGC14700.026.4486549
GTGTAAC7450.026.1040481
GGTATAC5750.025.0531621
GTATAAC4600.025.0531621
TCTCGCT15250.025.030252
GTGTTAC10550.023.2127631
CTCGCTC15900.023.0940573
GTACTAG5700.022.7456321
GTGTTAT10100.021.3944071
CTATACT6100.021.245644
GGAGTGC60700.021.232035
CGCTCTG15950.021.2160425
GTATAAT7150.021.1550311
Produced by FastQC (version 0.11.5)