FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3051149
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12800.037.264871
TCTGTCG13000.033.7824178
TCGCTCT14800.032.1060184
GTCTTGC30550.029.8074631
CTGTCGC16900.027.2644969
TCTCGCT16850.026.554392
GTATAAC6450.025.7711491
GTATTAT9000.025.696451
CGCTCTG16900.025.5604655
GTATAGG9050.025.554481
CTCGCTC18200.024.188173
GTATTAA7900.022.8706551
GTATATA12600.022.3696781
TTGCTCT41050.021.9230564
GTATTAG11550.021.9003851
GTGTACG5350.021.6138381
GTTTCAC46700.021.1713333
GTGTAGG20350.020.953341
GTGTTAG21100.020.5510711
GTGTAAA15900.020.4541321
Produced by FastQC (version 0.11.5)