FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3051149
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12750.046.303688
CTGTCGC18150.033.7174039
GTCTCGC15950.030.7015081
TCGCTCT17100.030.3143464
GTCTTGC28550.029.2592621
GGTATAC5950.027.8369941
GTATTAT8900.026.7014871
TCTCGCT18750.026.499082
CTCGCTC19150.025.5653653
CGCTCTG20100.025.0734335
GTCTCAC36450.024.3007161
GTCTTAC11750.023.902221
GTATTAG9050.023.8717441
GTATAAG9250.023.3555981
GTATATA12700.022.6812921
GTCGTAA1600.002263069622.5040931
GTATTGC8250.021.8221511
GGGTTTC51250.021.6390571
GTACGGA3004.86687E-721.6039281
TCTGTCA40250.021.4648178
Produced by FastQC (version 0.11.5)