FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2547560
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11100.040.8633048
GTCTCGC11150.040.1225431
GTCTTGC24050.033.3027081
TCTCGCT13800.031.8705352
TCGCTCT13200.031.6357784
CTGTCGC15950.030.6947549
GTGTACG3600.030.0649431
GTGTAAC7950.029.043871
GTATTAG7600.027.5331571
GTGTAAT9700.027.523371
CTCGCTC15050.027.3071843
GTGTTAC8900.026.7544211
GTATCGG1358.3140197E-426.7243961
GTATTAA5950.026.6794781
GGTATAC4650.026.3795621
GTATTAC5500.026.2384971
GTGTAGA12550.025.8726221
GTATAAT6650.024.9561631
CGCTCTG15050.024.8761275
GTATTAT7050.024.5636981
Produced by FastQC (version 0.11.5)