FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2547560
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11250.043.518668
GTCTCGC12900.039.633691
CTGTCGC14050.037.40819
TCGCTCT15150.032.791074
GTCTTGC23650.031.6663531
GTATTAT6400.030.379431
TCTCGCT16200.029.7815362
TATACTG6500.028.7991145
CTCGCTC15900.028.0746063
GTATAAA9350.027.7259671
CGCTCTG16250.027.026865
GTATAAC4550.026.9050241
GTCTTAC10000.026.6438871
GTATTAG7800.025.8499241
GTCGTAC1400.001040198325.7180391
GTATAAT5350.025.5738241
GTATTAA7000.024.6893161
GTTCTAA8650.024.142251
CTATACT6000.023.999264
GTGTTAG16250.022.600221
Produced by FastQC (version 0.11.5)