FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3473933
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16100.041.5826538
GTCTCGC15900.036.6953051
CTGTCGC18900.035.422269
TCGCTCT17350.034.022764
GTCTTGC31550.030.5135121
GTCGTAC1203.9212167E-430.38831
CCGTTCG2151.9428626E-830.1341679
GGTATAC6600.028.7307551
TCTCGCT20150.028.3251822
CTCGCTC21000.026.4906183
GTCTCAC41350.026.4565581
CGCTCTG21800.025.756885
GTACTAT7700.025.5735281
TTGCTCT45950.024.1262784
GTATAAT8500.024.0246331
GTCTTAC13700.023.955741
GTTTCAC52100.023.1579133
TTACTCT17750.022.7114494
TCTGTCA44800.022.656688
GTGTTAG23500.022.6554471
Produced by FastQC (version 0.11.5)