FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516080
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14400.034.493878
GTCTCGC13950.033.4936331
GTCTTGC32550.031.624571
GTATTAA10450.026.547511
CTGTCGC18550.026.0007679
TCGCTCT16200.025.7731974
GTGTAAG17850.024.130721
GTATAGA11250.024.0107481
GGTATAC6400.023.954981
TCTCGCT18150.023.8867572
GTATTAG10100.023.853331
CTCGCTC18100.023.553533
GTGTATA18750.022.5830841
GTATAAT11050.022.4632781
GTGTAGA16800.022.1624321
GTGTAAC10950.022.0017051
GTATTAT11050.021.8025931
GTCTTAC13800.021.6900841
TACCCGT3355.9382728E-821.4887185
GTGTTAG24850.021.446331
Produced by FastQC (version 0.11.5)